The OPI Drug Rapid Test is a lateral flow chromatographic immunoassay for the detection of Opiates in urine at a cut-off concentration of 2000ng/mL.
OPI Drug Rapid Test
1. Product Details
Product Name: The OPI Drug Rapid Test
Opiate refers to any drug that is derived from the opium poppy, including the natural products, Morphine and codeine, and the semi-synthetic drugs such as heroin. Opioid is more general, referring to any drug that acts on the opioid receptor.
Opioid analgesics comprise a large group of substance which control pain by depressing the central nervous system. Large doses of Morphine can produce highertolerance level and physiological dependency in users, and may lead to substance abuse. Morphine is excreted unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine is detectable in the urine for several days after an opiate dose.
The OPI Drug Rapid Test is a rapid urine screening test that can be performed without the use of an instrument. The test utilizes a monoclonal antibody to selectively detect elevated levels of Morphine in urine. The OPI Drug Rapid Test yields a positive result when the Morphine in urine exceeds 2000 ng/mL. This is the suggested screening cut-off for positive specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, USA).
The OPI Drug Rapid Test is an immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.
During testing, a urine specimen migrates upward by capillary action. Morphine, if present in the urine specimen below 2000 ng/mL, will not saturate the binding sites of the antibody in the test device. The Morphine conjugate will be captured by antibody and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Morphine level exceeds 2000 ng/mL because it will saturate all the binding sites of anti-Morphine antibodies.
A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen will generate a line in the test line region because of the absence of drug competition.
To serve as a procedural control, a colored line will always appear at the control line region, indicating that proper volume of specimen has been added and membrane wicking has occurred.
4. Directions of Test
Allow the test device, urine specimen and/or controls to reach room temperature (15-30°C) prior to testing.
(1)Bring the pouch to room temperature before opening it. Remove the test device from the sealed pouch and use it as soon as possible.
(2)Place the test device on a clean and level surface. Hold the dropper vertically and transfer 3 full drops of urine (approx. 100μl) to the specimen well (S) of the test device, and then start the timer. Avoid trapping air bubbles in the specimen well (S). See the illustration below.
(3)Wait for the colored line(s) to appear. The result should be read at 5minutes. Do not interpret the result after 10 minutes.
(Please refer to illustration above)
NEGATIVE: Two lines appear.
POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T).
(1)The OPI One Step Opiate Test Device provides only a qualitative, preliminary analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method.
(2) It is possible that technical or procedural errors, as well as other interfering substances in the urine specimen may cause erroneous results.
(3)Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results regardless of the analytical method used. If adulteration is suspected, the test should be repeated with another urine specimen.
(4)Certain medications containing opiate derivatives may produce a positive result. Additionally, foods and tea containing poppy products (the origin of the opiate) may also produce a positive result.
(5)A Positive Result does not indicate level of intoxication, administration route or concentration in urine.
Hangzhou Laihe Biotech Co., Ltd
Hangzhou Laihe Biotech Co., Ltd. (hereafter referred to as “Laihe Biotech”) is a life science company specialized in R & D, manufacturing, marketing and service in point-of-care testing, monitoring and health technology industry, is committed to provide fast, accurate and reliable health detection products and services.
Address: Room 521 Floor 5 & Floor 1,Building No. 2B,Tianhe High-Tech Park,No. 688,Bin'an Road,Changhe Sub-district,Binjiang District,Hangzhou City,Zhejiang Province,China