TCA Drug Rapid Test

TCA Drug Rapid Test
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Product Details

TCA Drug Rapid Test

1. Product Details

Name: The TCA Drug Rapid Test

Format: Cassette

Cut-off: 1000ng/mL(customized)

Specimen: Urine

Storage: 2-30°C

2. Summary

TCA (Tricyclic Antidepressants) are commonly used for the treatment of depressive disorders. TCA overdoses can result in profound central nervous system depression, cardiotoxicity and anticholinergic effects. TCA overdose is the most common cause of death from prescription drugs. TCAs are taken orally or sometimes by injection. TCAs are metabolized in the liver. Both TCAs and their metabolites are excreted in urine mostly in the form of metabolites for up to ten days. The TCA Drug Rapid Test is a rapid urine-screening test that can be performed without the use of an instrument. The test utilizes the antibody to selectively detect elevated levels of Tricyclic Antidepressants in urine.The TCA Drug Rapid Test yields a positive result when the Tricyclic Antidepressant in urine exceeds cut-off concentration.

3. Principle

The TCA Drug Rapid Test is a rapid chromatographic immunoassay based on the principle of competitive binding. Drugs which may be present in the urine specimen compete against the drug conjugate for binding sites on the antibody.

During testing, a urine specimen migrates upward by capillary action. Tricyclic Antidepressants, if present in the urine specimen below the cutoff level, will not saturate the binding sites of the antibody in the test strip. The antibodies will then be captured by immobilized Tricyclic Antidepressants conjugate and a visible colored line will show up in the test line region. The colored line will not form in the test line region if the Tricyclic Antidepressants level exceeds the cutoff level because it will saturate all the binding sites of anti-Tricyclic Antidepressants antibodies.

A drug-positive urine specimen will not generate a colored line in the test line region because of drug competition, while a drug-negative urine specimen or a specimen containing a drug concentration less than the cut-off will generate a line in the test line region. To serve as a procedural control, a colored line will always appear at the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.

4. Materials Provided

(1)Test device

(2)Disposable specimen dropper

(3)Package insert


5. Test Results

Please refer to the illustration above.

6. Effect of Urinary Specific Gravity

Fifteen (15) urine specimens with specific gravity range from 1.001 to 1.032 were spiked with 500 ng/mL and 1,500 ng/mL of Nortriptyline respectively. The TCA Drug Rapid Test was tested in duplicate using the fifteen neat and spiked urine specimens. The results demonstrate that varying ranges of urinary specific gravity does not affect the test results.

7. Effect of the Urinary pH

The pH of an aliquoted negative urine pool was adjusted to a pH range of 5 to 9 in 1 pH unit increments and spiked with Nortriptyline to 500 ng/mL and 1,500 ng/mL. The spiked, pH-adjusted urine was tested with the TCA Drug Rapid Test in duplicate. The results demonstrate that varying ranges of pH does not interfere with the performance of the test.

8. Cross-Reactivity

A study was conducted to determine the cross-reactivity of the test with compounds in either drug-free urine or Nortriptyline- positive urine. The following compounds show no cross-reactivity when tested with the TCA Drug Rapid Test at a concentration of 100 ng/mL.

Hangzhou Laihe Biotech Co., Ltd

Address: Room 521 Floor 5 & Floor 1,Building No. 2B,Tianhe High-Tech Park,No. 688,Bin'an Road,Changhe Sub-district,Binjiang District,Hangzhou City,Zhejiang Province,China

Contact: Helen

Tel: +86-571-86658003

Fax: +86-571-86658000