Malaria Pf/Pan Rapid Test Cassette
1. Product Details
Test Name: The Malaria Pf/Pan Rapid Test Cassette
Test Item: Circulating antigens of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae
Specimen: Whole Blood
The Malaria Pf/Pan Rapid Test (Whole Blood) is a qualitative, membrane based immunoassay for the detection of P.f, P.v, P.o and P.m antigens in whole blood. The membrane is pre-coated with anti-HRP-II antibodies and anti-Aldolase antibodies. During testing, the whole blood specimen reacts with the dye conjugate, which has been pre-coated on the test strip. The mixture then migrates upward on the membrane by capillary action, reacts with anti-Histidine-Rich Protein II (HRP-II) antibodies on the membrane on P.f Test Line region and with anti-Aldolase antibodies on the membrane on Pan Line region. If the specimen contains HRP-II or Plasmodium-specific Aldolase or both, a colored line will appear in P.f line region or Pan line region or two colored lines will appear in P.f line region and Pan line region. The absence of the colored lines in P.f line region or Pan line region indicates that the specimen does not contain HRP-II and/or Plasmodium-specific Aldolase.
To serve as a procedure control, a colored line will always appear in the control line region indicating that proper volume of specimen has been added and membrane wicking has occurred.
3. Directions for Use
Allow the test device, specimen, buffer, and/or controls to equilibrate to room temperature (15-30°C)priorto testing.
(1) Remove the test device from the foil pouch and use it as soon as possible. Best resultswill be obtained if the assay is performed within one hour.
(2) Place the test device on a clean and level surface. Transfer the specimen by a pipette or a dropper:
To use a Pipette: Transfer 10 μL of whole blood to Well-1 (W1) of the test device, then add 3 full drops of buffer to Well-2 (W2), and start the timer. (See illustration ① below).
Avoid trapping air bubbles in W1. At the end of 5 minutes, add 1 full drop of buffer to the W1 (See illustration ③ below).
To use a Disposable Specimen Dropper: Hold the dropper vertically; draw the specimen up to the Fill Line as shown in illustration ① below. Transfer the specimen to W1of the test device, then add 3 full drops of buffer to W2 and start the timer. Avoid trapping air bubbles in W1. At the end of 5 minutes, add 1 full drop of buffer to W1 (See illustration ③ below).
3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret the result after 20 minutes.
Hangzhou Laihe Biotech Co., Ltd
Address: Room 521 Floor 5 & Floor 1,Building No. 2B,Tianhe High-Tech Park,No. 688,Bin'an Road,Changhe Sub-district,Binjiang District,Hangzhou City,Zhejiang Province,China