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The Definition of Colloidal Gold Method

Apr 24, 2018

Colloidal gold is a commonly used labeling technique. It is a new type of immunolabeling technology that uses colloidal gold as a marker to apply antigens and antibodies. It has unique advantages. It has been widely used in various biological studies in recent years.  At the same time in the flow, electron microscope, immune, molecular biology and even biochips may use this technique.

The specific antigen or antibody is fixed on the membrane in strips, and the colloidal gold labeling reagent (antibody or monoclonal antibody) is adsorbed on the binding pad. When the sample to be tested is added to the sample pad at one end of the test strip, the capillary action is performed. After moving forward and dissolving colloidal gold labeling reagents on the binding pad to react with each other, and then moving to a fixed antigen or antibody area, the combination of the test substance and the gold labeling reagent is specifically bound to it and is retained. On the test strip, the color development result can be observed with the naked eye.