The anti-Chlamydia lipopolysaccharide monoclonal antibody and the goat anti-mouse IgG polyclonal antibody were respectively immobilized on the solid phase nitrocellulose membrane, and were colloidally labeled with another anti-Chlamydia lipopolysaccharide monoclonal antibody and other reagents and raw materials. Colloidal gold immunochromatography, a chlamydial assay established in the form of a double-antibody sandwich, was used for the detection of chlamydia in female urethra and in the female cervix. In order to achieve the detection of female cervix and male urethra in the presence of chlamydia, clinical diagnosis of chlamydia infection, the test results also require the clinician to combine the patient's symptoms, signs and other test results to further determine the purpose. The gold standard legal test for Chlamydia test kit has the advantages of rapidity, convenience, and high accuracy. It will save a lot of time for the clinician's auxiliary diagnosis.