Tumor marker detection kit
A. tumor marker and its progress
In recent years, tumor markers (oncogene and tumor suppressor gene and tumor-related genes and their products, circulating nucleic acids (DNA and mRNA) markers) into the molecular and genetic detection and application level. Become the focus of research on tumor markers proteomics. Serum tumor markers have become important indicators of laboratory examination of patients with tumors (table 5).
B. tumor marker of the joint inspection
Due to the lack of tissue-specific tumor markers, several markers of the same tumor can occur, and the tumor may appear with a marker. To improve the clinical value of tumor markers, according to different characteristics and different biomarkers of tumor, reasonable to select multiple markers are very important. Should pay attention to is that joint detection improves sensitivity, specificity decreases, so you should select specific markers are combined. Many clinical value of tumor marker is not clear, and the lack of standardized reagents, quality control standards and determination methods, without selective markers in checks, will not only fail to improve efficiency, will also increase the cost. Should select the best common tumor markers combination should be used for clinical diagnosis (table 6).
C ... malignant tumors circulating nucleic acids of genetic markers
Circulating nucleic acids are present in the peripheral blood of free DNA and mRNA. Studies confirm that in recent years, circular DNA and mRNA in cancer patients with tumor characteristics, application of PCR or RT-PCR for detecting circulating nucleic acids on tumor micrometastasis in early diagnosis and monitoring of important value. Compared with the traditional tumor-associated Antigen, circulating nucleic acids detection high sensitivity and specificity.
D. detection and analysis of Proteomics of tumor markers
Proteomics to high-throughput, automated tumor protein expression profiling analysis helps to find specific molecular markers in early diagnosis of tumors. Current proteomics technologies have two electrophoresis, matrix-assisted laser desorption ionization-time of flight mass spectrometry and surface-enhanced laser desorption ionization time of flight mass spectrometry (seldi-TOF-MS), fast, sensitive, high throughput, automated analysis of changes of serum protein patterns in, has been used to detect prostate cancer, ovarian cancer, pancreatic cancer, breast cancer, colorectal cancer, and so on.
Our country has developed multi-tumor markers protein chip, using the principle of micro-arrays, using chemiluminescent immunoassay membrane technology and computer image analysis, high-throughput of multiple tumor markers combined detection and comprehensive analysis, parallelism, fast and simultaneous detection of multiple tumor markers, significantly improves diagnostic sensitivity and specificity. Clinical application of multi-tumor markers protein chip for detecting ovarian cancer, colorectal cancer, lung cancer diagnosis rate amounted to 90%, 85% and 80%. Cancer patient observation, monitoring of metastasis and recurrence also has a high value.